Below share some information:
https://www.biorxiv.org/content/10.1101 ... l.pdf+html
Below product:
https://www.inno-maker.com/product/cam- ... w-trigger/
Video Compare for different sensor module like imx477,imx462,imx327,imx290...etc :
https://drive.google.com/drive/folders/ ... gz9IVdKlC_
Overview:
Observing the activity of large populations of neurons in vivo is critical for understanding brain function and
dysfunction. The use of fluorescent genetically-encoded calcium indicators (GECIs) in conjunction with
miniaturized microscopes is an exciting emerging toolset for recording neural activity in unrestrained animals.
Despite their potential, current miniaturized microscope designs are limited by using image sensors with low
frame rates, sensitivity, and resolution. Beyond GECIs, there are many neuroscience applications which would
benefit from the use of other emerging neural indicators, such as fluorescent genetically-encoded voltage
indicators (GEVIs) that have faster temporal resolution to match neuron spiking, yet, require imaging at high
speeds to properly sample the activity-dependent signals. We integrated an advanced CMOS image sensor
into a popular open-source miniaturized microscope platform. MiniFAST is a fast and sensitive miniaturized
microscope capable of 1080p video, 1.5 µm resolution, frame rates up to 500 Hz and high gain ability (up to 70
dB) to image in extremely low light conditions. We report results of high speed 500 Hz in vitro imaging of a
GEVI and ~300 Hz in vivo imaging of transgenic Thy1-GCaMP6f mice. Finally, we show the potential for a
reduction in photobleaching by using high gain imaging with ultra-low excitation light power (0.05 mW) at 60
Hz frame rates while still resolving Ca2+ spiking activity. Our results extend miniaturized microscope
capabilities in high-speed imaging, high sensitivity and increased resolution opening the door for the opensource community to use fast and dim neural indicator
https://www.biorxiv.org/content/10.1101 ... l.pdf+html
Below product:
https://www.inno-maker.com/product/cam- ... w-trigger/
Video Compare for different sensor module like imx477,imx462,imx327,imx290...etc :
https://drive.google.com/drive/folders/ ... gz9IVdKlC_
Overview:
Observing the activity of large populations of neurons in vivo is critical for understanding brain function and
dysfunction. The use of fluorescent genetically-encoded calcium indicators (GECIs) in conjunction with
miniaturized microscopes is an exciting emerging toolset for recording neural activity in unrestrained animals.
Despite their potential, current miniaturized microscope designs are limited by using image sensors with low
frame rates, sensitivity, and resolution. Beyond GECIs, there are many neuroscience applications which would
benefit from the use of other emerging neural indicators, such as fluorescent genetically-encoded voltage
indicators (GEVIs) that have faster temporal resolution to match neuron spiking, yet, require imaging at high
speeds to properly sample the activity-dependent signals. We integrated an advanced CMOS image sensor
into a popular open-source miniaturized microscope platform. MiniFAST is a fast and sensitive miniaturized
microscope capable of 1080p video, 1.5 µm resolution, frame rates up to 500 Hz and high gain ability (up to 70
dB) to image in extremely low light conditions. We report results of high speed 500 Hz in vitro imaging of a
GEVI and ~300 Hz in vivo imaging of transgenic Thy1-GCaMP6f mice. Finally, we show the potential for a
reduction in photobleaching by using high gain imaging with ultra-low excitation light power (0.05 mW) at 60
Hz frame rates while still resolving Ca2+ spiking activity. Our results extend miniaturized microscope
capabilities in high-speed imaging, high sensitivity and increased resolution opening the door for the opensource community to use fast and dim neural indicator
Statistics: Posted by joe-zou — Wed Jan 10, 2024 4:41 am